Absolutely gender of voice makes a difference.
In general, both men and women respond better to a female speaker's voice, although I have found that using both at once has an effect in a different direction that overrules and overpowers any lesser effect adding in a male voice might have, so I use both at once.
EDIT: Unless the sub is mono-gender usage only, and then in some cases, that gender of voice will be the only one used, depending on the goals of the sub.
In general, both men and women respond better to a female speaker's voice, although I have found that using both at once has an effect in a different direction that overrules and overpowers any lesser effect adding in a male voice might have, so I use both at once.
EDIT: Unless the sub is mono-gender usage only, and then in some cases, that gender of voice will be the only one used, depending on the goals of the sub.
Subliminal Audio Specialist & Administrator
The scientist has a question to find an answer for. The pseudo-scientist has an answer to find a question for. ~ "Failure is the path of least persistence." - Chinese Fortune Cookie ~ Logic left. Emotion right. But thinking, straight ahead. ~ Sperate supra omnia in valorem. (The value of trust is above all else.) ~ Meowsomeness!
The scientist has a question to find an answer for. The pseudo-scientist has an answer to find a question for. ~ "Failure is the path of least persistence." - Chinese Fortune Cookie ~ Logic left. Emotion right. But thinking, straight ahead. ~ Sperate supra omnia in valorem. (The value of trust is above all else.) ~ Meowsomeness!